TABLE 4.

Results from RT-PCR detection of ALS gene expression in C. albicans-inoculated RHVE specimensa

Time (h) or % positive signalStrainRT-PCR result
ALS1ALS2ALS3ALS4ALS5ALS6ALS7ALS9
Inoculum culture3153A++++++W+
GC2+++++++
GC8++++++++
123153A+/++/++/++/−+/+−/+−/−+/+
24+/++/++/+W/+W/+W/+W/+W/+
36+/++/++/++/++/++/+−/W+/+
12GC2+/++/++/+−/−W/WW/W+/++/+
24+/++/++/+−/++/++/W−/−+/+
36+/++/++/++/++/++/++/++/+
12GC8+/++/++/+W/W+/+W/W+/W+/+
24+/++/++/++/++/++/++/++/+
36+/++/++/++/++/+−/W+/++/+
% Positive signal1001001006183506194
  • a Results are reported as + for a strong positive signal, W for a weak signal, and − for samples that fell below the detection limit of the assay (9). Duplicate samples are indicated for each time point in the RHE analysis.