TABLE 1.

Plasmids used in this study

PlasmidProtein encodedReference
pWPsf4None12a
pWPsf4DIpaDThis work
pWPsf4D6IpaDΔ1-20This work
pWPsf4D910IpaDΔ41-80This work
pWPsf4D1112IpaDΔ81-120This work
pWPsf4D1314IpaDΔ121-160This work
pWPsf4D1516IpaDΔ161-180This work
pWPsf4D1718IpaDΔ201-240This work
pWPsf4D1920IpaDΔ241-280This work
pWPsf4D2122IpaDΔ281-320This work
pWPsf4D23EIpaDΔ321-332This work
pWPsf4D5EIpaDΔ328-332This work
pWPsf4DcysIpaDSC321ASThis work
pWPsfcIpaC30
pKPL103SipD28
  • a pWPsf4 was derived from pUC18 for specific expression of ipa genes in S. flexneri as described by Harrington et al. (12).