TABLE 2.

Patient demographic information and ALS RT-PCR results for human clinical specimensa

Sample no.b or % detectionAge (yr)PregnantSymptomaticEstrogen supplementdRT-PCR of 2 μg of total RNAPoly(A) RNA used in RT-PCR analysis (μg)RT-PCR of poly(A) RNA
ALS1ALS2ALS3ALS4ALS5ALS6ALS7ALS9ALS1ALS2ALS3ALS4ALS5ALS6ALS7ALS9
Sample no.
    2316NoNo1.0++++
    4518NoNoAlesseNRNRNRNRNRNRNRNR2.0+++++++
    3023NoNoNRNRNRNRNRNRNRNR1.8+++WW
    847NoNo++++++2.0+++W++
    3618YesNo++1.3+++
    2822YesNoNRNRNRNRNRNRNRNR1.1+
    3224YesNoNRNRNRNRNRNRNRNR2.0+++
    3324YesNoOrtho-Tri-Cyclen+++W+2.0++++++
    2227YesNo+++++2.0+++++++
    221NoYesOrtho-Tri-Cyclen+2.0
    622NoYes+1.2++++
    2425NoYes+1.5++++
    3126NoYesLoestrin 1/20W+1.3+
    2929NoYesClomid++2.0++++W+
    2734NoYesNordette++W+1.7++++++
    4140NoYesPremarin++++++1.2WWW
    746NoYes++2.0+++++++
    5c57NoYesPrempro2.0+
    4218YesYes++++1.1++++
    1522YesYes++++++2.0++++
    4022YesYes2.0
    2123YesYes++WW+2.0+++WW+
    3926YesYes++W0.8++++
% Detection474753054732588778703026132670
    Pregnant8080802020202060
    Nonpregnant927762383183177
    Symptomatic8671572121142164
    Asymptomatic8989894433113378
  • a Results are reported as + for a strong positive signal and W for a weak positive signal. A blank space designates a sample that fell below the detection limit of the assay (9). Samples that were not run are designated as NR.

  • b C. albicans strains isolated from these clinical samples used the sample number preceeded by GC.

  • c C. albicans and C. parapsilosis were recovered from this specimen.

  • d Generic names for the estrogen supplements are as follows: for Alesse and Nordette, ethinyl estradiol and levonorgestrel; for Ortho Tri-Cyclen, ethinyl estradiol and norgestimate; for Loestrin 1/20, ethinyl estradiol and norethindrone; for Clomid, clomiphene citrate; for Premarin, conjugated estrogens; and for Prempro, conjugated estrogens and medroxyprogesterone acetate.