TABLE 3.

ALS RT-PCR results for murine model specimensa

Time or % detectionStrainMouse no.Total RNA (ng)RT-PCR result
ALS1ALS2ALS3ALS4ALS5ALS6ALS7ALS9
Time
    Inoculum3153A+++++++
    GC2++++++W+
    GC8+++++++
    Day 43153A1400++W+
260++
3700
GC21130+
2270++++
3800++
4150++WW+
GC81220++++
2210
3290W+W+
    Day 73153A1220++++W++
2500++W+
GC21220+++W
2280+++
GC81590+++
% Detection
    Overall80736777201347
    Day 470605000201050
    Day 71001001002020202040
  • a Results are reported as + for a strong positive signal and W for a weak positive signal. A blank space designates a sample that fell below the detection limit of the assay (9).