Table 2.

In vivo depletion of T-cell subpopulations in C. trachomatis-infected B6 and B-cell deficient micea

In vivo treatmentbB6 miceB-cell-deficient mice
Day 3Day 7Day 3Day 7
BloodSpleenBloodSpleenBloodSpleenBloodSpleen
CD4CD8CD4CD8CD4CD8CD4CD8CD4CD8CD4CD8CD4CD8CD4CD8
Anti-CD4015017019015132030222041
Anti-CD8301270272250600500550481
  • a Groups of B6 and B-cell-deficient mice that had resolved a primary C. trachomatis genital tract infection were injected i.p. with either anti-CD4 or anti-CD8 antibody as described in Materials and Methods. Mice were administered a secondary infectious challenge on day 56 post primary infection. Anti-CD4 or anti-CD8 antibody was administered on days 50, 51, 52, 55, 58, 61, 64, 67, 70, and 73. Mean percentages of cells that stained positively for CD4 or CD8 in the peripheral blood and in the splenocyte population of antibody-treated mice at 3 and 7 days post secondary infectious challenge are shown (three mice per determination).

  • b Treatment of B6 or B-cell-deficient mice with buffer or rat Ig did not affect the distribution of CD4+ or CD8+ T cells in the peripheral blood or splenocyte population. The proportions of CD4+ and CD8+ T cells in the peripheral blood and spleens of infected B6 mice treated with either buffer or rat Ig were approximately 30 and 18%, respectively. For infected B-cell-deficient mice, the percentages of CD4+ and CD8+ T cells were approximately 42 and 35%, respectively.