Table 1.

Secretion of IL-12 by DC with various stimulia

Treatment of iDCSecretion (pg/ml) of:
IL-12 (p40 + p70)IL-12 (p70)
IL-4 + GM-CSF17 ± 23.8 ± 0.1
GM-CSF alone51 ± 54.2 ± 0.1
TNF-α + GM-CSF4,900 ± 1308.3 ± 0.1
IL-1β + GM-CSF32,250 ± 1,2007.0 ± 0.1
LPS + GM-CSF130,000 ± 6,000653 ± 52
Emulsion buffer + GM-CSF14 ± 42.9 ± 0.1
BCG-CWS + GM-CSF5,400 ± 2106.4 ± 0.1
  • a iDC differentiated with GM-CSF and IL-4 for 6 days were incubated for 2 days in medium containing GM-CSF (500 IU/ml) and the following material: IL-4 (100 IU/ml), GM-CSF alone, TNF-α (100 IU/ml), IL-1β (100 ng/ml), LPS (10 ng/ml), emulsion buffer (15 μl/ml), or BCG-CWS (15 μg/ml). The culture supernatants were collected, cleared by centrifugation, and measured by ELISA kits. Values are the means ± SD of triplicate determinations. The experiments were repeated twice, and results of one experiment are shown.