Table 2.

Oligonucleotide primers used in this study

PrimerSequenceaAnnealing sitea
Coding strand
 Donne-65′-TCTTGGTGCTTGCGTGTC-3′19–208
 Donne-285′-CGCGGATCCATGGTTTCTAAAATCATGAAT-3′274–294
 Donne-3625′-AGGTCTGTCTTTGATTGAA-3′309–327
 Donne-4235′-GATTATTCCGTGACCTATT-3′223–241
Noncoding strand
 Donne-265′-GTAAGCGTCAGATAGTAACCAA-3′638–617
 Donne-295′-GCGAAGCTTTTACTTCATAAAATATGTAAC-3′855–835
 Donne-3635′-CCTGAGTAAAACAGGATAC-3′953–935
 Donne-3825′-TCCTTCGGGTGATTGTGTA-3′1300–1282
 Donne-4165′-GTTGCGCTCATTACTTCT-3′443–426
  • a The underlined portion of each primer corresponds to the annealing site indicated within the bfpcluster nucleotide sequence (EMBL accession number Z68186).