Table 1.

Strains and plasmids used

Strain or plasmidRelevant phenotype(s)Source or reference
E. coli strains
 SM10λpirthi recA thr leu tonA lacY supELaboratory strain
RP4-2-Tc::Mu λ::pir strain
 AC-E184SM10λpir(pAC160)5
 AC-E189SM10λpir(pAC214)5
 DSM-E713SM10λpir(pDSM375)17
V. cholerae strains
 C6709-1El Tor biotype, SmrRoberts et al.a
 AC-V168C6709-1 lacZ::pGP704, LacZ5
 DSM-V382C6709-1rpoS::pGP70417
 DSM-V490C6709-1 rpoS::pCVD442This work
 DSM-V491C6709-1 ΔrpoS17
 DSM-V506C6709-1 ΔrpoS (pFY7)This work
 DSM-V583C6709-1(pMMB67EH), LacZThis work
 DSM-V714C6709-1(pFY7)This work
 DSM-V717DSM-V506 cured of pFY7This work
 DSM-V719DSM-V491 reverted to wild typeThis work
 DSM-V720DSM-V717 reverted to wild typeThis work
Plasmids
 pAC160pGP704::′iviVI5
 pAC214pGP704::′orf35
 pFY7pMMB67EH::′nlpD-rpoS-mutS22
 pDSM375pGP704::′rpoS17
 pDSM747pCVD442::′nlpD-rpoS-mutSThis work
 pMMB67EHIncQ broad-host-range cloning vector22
  • a A. Roberts, G. D. Pearson, and J. J. Mekalanos, Proc. 28th Joint Conf., U.S.-Japan Cooperative Med. Sci. Prog. Cholera Related Diarrheal Dis., p. 43–47, 1992.