Table 2.

Immune response to LPS and water extract in mice immunized with invaplex 24 or invaplex 50 from S. flexneri 5

ImmunizationDayMedian ELISA reading (range)
S. flexneri 5 LPSWater cxtract
Vir+Vir
IgAIgGIgAIgGIgAIgG
Invaplex 24 00.036 (0.035–0.037) 0.026 (0.025–0.038) 0.031 (0.03–0.033) 0.020 (0.019–0.022) 0.040 (0.034–0.042) 0.023 (0.021–0.023)
280.038 (0.035–0.050) 0.036 (0.026–0.071) 0.097 (0.084–0.163)*0.136 (0.113–0.137)*0.035 (0.035–0.042) 0.030 (0.025–0.051)*
420.056 (0.038–0.245) 0.077 (0.059–0.238)*0.598 (0.408–0.692)*0.827 (0.487–0.957)*0.043 (0.039–0.135) 0.059 (0.050–0.259)*
Invaplex 50 00.033 (0.033–0.037) 0.027 (0.026–0.034)0.044 (0.040–0.045) 0.020 (0.020–0.022) 0.046 (0.044–0.046) 0.023 (0.022–0.024)
280.045 (0.043–0.121)*0.056 (0.032–0.174)*0.054 (0.049–0.066)*0.075 (0.060–0.095)*0.069 (0.060–0.087)*0.112 (0.085–0.192)*
421.415 (0.307–1.821)*1.315 (0.455–1.554)*0.286 (0.189–0.882)*0.351 (0.276–0.548)*0.603 (0.373–0.954)*0.555 (0.411–0.725)*
  • a Mice were immunized intranasally three times with S. flexneri 5-derived invaplex 24 or invaplex 50. The serum IgA or IgG reactive with S. flexneri 5 LPS and water extract (Vir+ or Vir) was determined by ELISA. Blood was taken from all mice before treatment (day 0), after the second immunization (day 28), and after three immunizations (day 42). Values represent the median (25/75 percentile) for each group of mice (n = 5). Values significantly greater (P < 0.05, Wilcoxon signed rank test) than for the day 0 serum are indicated by asterisks. Sera were diluted 1/180 for IgA analysis and 1/360 for IgG analysis.