Table 1.

Bacterial strains and plasmids used and constructed in this study

Strain or plasmidRelevant characteristic(s)aSource or reference
Strains
P. gingivalis33277Type strain from ATCCThis laboratory
P. gingivalisUPFDerivative of 33277,fimAp ::lacZ gene, Emr 31
P. gingivalis MPF35ADerivative of P. gingivalis 33277,mpf35::fimA,fimAp ::lacZ gene, Emr This study
P. gingivalisMP150ADerivative of P. gingivalis 33277,mp150::fimA,fimAp ::lacZ gene, Emr This study
P. gingivalisMPS10ADerivative of P. gingivalis 33277,mps10::fimA,fimAp ::lacZ gene, Emr This study
P. gingivalisYPF1FimA-deficient mutant of 33277, Emr 13
P. gingivalis YPP1RgpA-deficient mutant of 33277, Emr 23
P. gingivalis YPP2Kgp-deficient mutant of 33277, Emr 23
E. coliDH5α endA1 hsdR17 supE44 thi-1 recA gyrA96 relA1Δ(lacZYA-argF) U169λ-φ80 dlacZΔM15, recipient for recombinant plasmidsGibco-BRL
Plasmids
 pJRD215Wide-host-range cosmid vector, Kmr Smr Mob+, unable to replicate in P. gingivalis 22
 Tn4351 Contains a 3.8-kb EcoRI fragment from pBF4, Tcr expressed in E. coli, Emr expressed in P. gingivalis 24
 R751IncP plasmid used to mobilize vectors from E. coli toBacteroides recipient TprTra+ 22
 pMP58 fimAp ::lacZgene in pUC19 with a 16-bp deletion from −23 to −8 (mp58::lacZ), Amr 31
 pMPF35 mpf35::lacZ gene in pJRD215, three-base change from TTG to CCA at position −46 to −44, Kmr 31
 pMP150 mp150::lacZ gene in pJRD215, 150-bp deletion from −240 to −90, Kmr 31
 pMPS10 mps10::lacZ gene in pJRD215, three-base change from TAA to GCC at position −11 to −9, Kmr 31
 pMP591 mp150::lacZ gene in pJRD215, 15-bp deletion from −85 to −71 and 17-bp deletion from −64 to −48, Kmr 31
  • a Abbreviations: Kmr, Smr, Tcr, Emr, and Tpr, resistance to kanamycin, streptomycin, tetracycline, erythromycin, and trimethoprim, respectively; Mob+, can be mobilized; Tra+, capable of self transfer;fimA p, fimA promoter.