Table 1.

Inhibition of O2 release by human neutrophils (donor 5) in response to PMA by the HGE agent

TreatmentaReduction of ferricytochrome c (nmol of O2/106 neutrophils/2 h)b
−SOD+SOD
Medium control2.4 ± 0.51.5 ± 0.0
PMA13.8 ± 2.0* 0.8 ± 1.3
HL-60 lysate1.3 ± 0.62.5 ± 0.6
HL-60 lysate + PMA11.1 ± 0.5* 0.0 ± 0.0
HGE agent0.0 ± 0.00.0 ± 0.0
HGE agent + PMA1.0 ± 0.90.0 ± 0.0
  • a Neutrophils from donor 5 were incubated for 30 min with medium, uninfected HL-60 lysate, or host cell-free HGE agent prior to PMA stimulation in the presence or absence of SOD.

  • b Mean ± standard deviation (n = 3). Results are representative of three independent experiments. ∗, P < 0.01 compared to neutrophil-alone (no PMA) control (Student's t test).