TABLE 1.

Strains and plasmids used in the present study

Strain or plasmidDescriptionSource or reference
Strains
    E. coli
        HB101 proA2 leuB6 thi-1 lacY1 hsdR hsdM recA13 supE44 rpsL20 34
        JM109F′ traD36 lac1q Δ(lacZ) M15 proA+B+/el4 (McrA) Δ(lac-proAB) thi gyraA96(Na1r) endA1 hsdR17(rk mk+) relA1 supE44 recA1 34
    P. aeruginosa
        NH57388AStable mucoid CF mouse sputum isolate, hyperproducing alginate, functional AHL-based QS, mutation in mucAThis study
        NH57388BNonmucoid isolate from the same CF mouse sputum sample, nonfunctional AHL-based QSThis study
        NH57388CNonmucoid isolate, derived in vitro from mucoid NH57388A, functional AHL-based QS, mutation in algTThis study
        PAO1Prototypic nonmucoid wild-type, functional AHL-based QSB. H. Iglewski
Plasmids
    pRK600Cmr; ori, ColE1 RK2-Mob+ RK2-Tra+ 28
    pCD100 FtraD36 lacIq (lacZ)M15 proA+B+/el4 (McrA)(lacproAB)thi gyr; AlgTmucA(−)BCD operonM. Kalai