TABLE 2.

Mortality recorded after intratracheal P. aeruginosa challenge of CF mice and BALB/c mice with the mucoid strain +QS (NH57388A), the nonmucoid strain −QS (NH57388B), and the nonmucoid strain +QS (NH57388C)a

ParameterExpt 1Expt 2Expt 3Expt 4
CF mice (mucoid +QS NH57388A)BALB/c mice (mucoid +QS NH57388A)BALB/c mice (mucoid +QS NH57388A)BALB/c mice (nonmucoid −QS NH57388B)BALB/c mice (mucoid +QS NH57388A)CF mice (mucoid +QS NH57388A)CF mice (nonmucoid +QS NH57388C)CF mice (nonmucoid −QS NH57388B)
Mortality (no. of animals dying/total no. tested) at day:
    11/260/151/221/151/200/132/151/13
    23/260/151/221/151/202/133/150/13
    34/260/154/221/151/203/132/151/13
    44/260/153/220/151/200/131/150/13
    55/260/152/220/150/201/131/150/13
    60/260/151/220/150/200/130/151/13
    70/260/150/220/150/200/130/150/13
Total17/260/1512/223/154/206/139/153/13
Mortality (%)65***055*20204660**23
95% Confidence interval4-830-2232-764-486-4419-7532-845-54
  • a Expt 1, inoculum of 5 × 106 CFU/lung (bacteria were instilled with crude alginate); Expt 2, inoculum of 5 × 107 CFU/lung (bacteria were instilled with crude alginate); Expt 3, inoculum of 5 × 107 CFU/lung (bacteria were instilled with purified alginate); Expt 4, inoculum of 5 × 106 CFU/ml (bacteria were instilled with purified alginate).

  • b * and **, P < 0.05 compared to strain NH57388B resuspended in culture supernatant (crude alginate [*]) or in purified alginate (**); ***, P < 0.001 compared to BALB/c mice.