TABLE 1.

Bacterial strains

StrainRelevant characteristic(s)Reference
K. pneumoniae strains
    C105K35 capsule49
    C105NCVNoncapsulated variant of C10549
    PKL1019C105 containing pACYC184This study
    PKL1024C105NCV containing pACYC184This study
    RMV22C105 containing pRMV1 (TibA+)This study
    RMV23C105NCV containing pRMV1 (TibA+)This study
E. coli strains
    MG1655E. coli K-12 reference strain1
    MS427MG1655 Δflu23
    MS528MG1655 Δflu Δfim23
    OS56MS427 attB::bla-PA1/04/03-gfpmut3b*-T0 (Gfp+)This study
    OS60MS427 containing pACYC184 and pBADThis study
    OS70OS56 containing pACYC184 (Gfp+)This study
    OS82MS427 containing pAR163 (DsRed:T3+)This study
    OS108OS56 containing pRMV1 (Gfp+ TibA+)This study
    OS109OS82 containing pRMV1 (DsRed:T3+ TibA+)This study
    OS136OS56 containing pRMV4b (Gfp+ TibCA+)This study
    RMV3MS427 containing pRMV1 (TibA+)This study
    RMV4MS427 containing pRMV1 and pPKL4 (TibA+ Fim+)This study
    RMV6MS427 containing pRMV1 and pOS38 (TibA+ TibC+)This study
    RMV7MS427 containing pRMV1 and pBR322 (TibA+)This study
    RMV8MS427 containing pRMV1 and pBAD (TibA+)This study
    RMV10MS427 containing pACYC184 and pPKL4 (Fim+)This study
    RMV11MS427 containing pACYC184 and pBR322This study
    RMV12MS427 containing pACYC184 and pOS38 (TibC+)This study
    RMV19MS528 containing pMW119This study
    RMV20MS528 containing pRMV3 (TibA+)This study
    RMV24MS528 containing pACYC184 and pBR322This study
    RMV25MS528 containing pRMV1 and pBR322 (TibA+)This study
    RMV26MS528 containing pACYC184 and pKT274 (K1+)This study
    RMV27MS528 containing pRMV1 and pKT274 (TibA+ K1+)This study