TABLE 2.

Plasmids used in this study

PlasmidRelevant genotype or phenotypeReference or source
pPKL4fim gene cluster from E. coli PC31 in pBR32225
pRL27Mini-Tn5 delivery vector, source for PtetA28
pSM1690PrrnB,P1-RBSII-gfpmut3b+-T0-T1 NotI fragment in LOW2, Kmr48
pTP801cat gene in BamHI site of pUC1935
pTP809pBR322 derivative with multiple cloning site introduced between EcoRI and NdeI sites35
pAR90cfp* inserted between SphI and HindIII sites in pJBA2742
pDsRed.T3Encodes a rapidly maturating DsRed.T3 variant4
pKKJ128flu gene from E. coli MG1655 in pACYC18422
pKT274E. coli K1 capsule operon in pHC7944
pLDR8Temperature-sensitive helper plasmid carring the λ int gene11
pLDR11Vector containing the attP sequence for integrating DNA into the lambda attachment site attB11
pMW119Low-copy-number cloning vectorNippon Gene
pAR81cat gene from pTP801 ligated into BamHI-digested pTP809, CmrThis study
pAR94PrrnB,P1 containing SacI-XbaI fragment of pSM1690 inserted into pAR90 digested with SacI and XbaIThis study
pAR163Amplification of dsred2.T3 with primers Dsred2d and UDsRed2.T3 and ligation into XbaI-HindIII-digested pAR94; results in replacement of cfp* with dsred2.T3, controlled by PrrnB,P1This study
pAR176Amplification of PtetA with primers ar047 and ar048 from pRL27 and ligation into SacI-XbaI-digested pSM1690; results in replacement of PrrnB,P1 with PtetAThis study
pAR178Amplification of cat gene with primers ar059 and ar060 from pAR81 and ligation with ClaI-SalI-digested p15A ori-containing fragment from pSM1690 (amplified by PCR with primers ar057 and ar058)This study
pAR179Insertion of PrrnB,P1-RBSII-dsred2.T3-T0 containing NotI cassette from pAR163 intoThis study
the NotI site of pAR178
pOS32gfpmut3b gene from plasmid pAR176 ligated to XbaI-HindIII-digested pAR179; construct has gfpmut3b gene under transcriptional control of the E. coli rrnB-P1 promoterThis study
pOS38tibC gene from E. coli H10407 PCR amplified with primers 531 and 532 and cloned into XhoI-KpnI-digested pBADThis study
pRMV1tibA gene from E. coli H10407 PCR amplified with primers 535 and 536 and cloned into BamHI-XmaIII-digested pACYC184This study
pRMV3tibA gene from E. coli H10407 PCR amplified with primers 563 and 564 and cloned into HindIII-BamHI-digested pMW119This study
pRMV4btibC and tibA genes from E. coli H10407 PCR amplified with primers 573 and 536 and cloned into BamHI-XmaIII-digested pACYC184This study