TABLE 3.

Expression of P. gingivalis genes induced during 3 h of contact with HEp-2 epithelial cells

ORFGeneFold increase measured by
Microarray analysisNorthern blot analysisaQRT-PCRb
PG0045htpG6.262.5
PG0520groEL11.11055.1
PG0521groES5.510.4
PG0593htrA3.728.5714.4
PG1089rprY3.3411.631.6
PG1208dnaK3.3813.7
PG1240tetR7.91.8644.4
16S rRNA1.031
  • a Signal intensities were measured with an Alpha Imager 2200 (Alpha Innotech Corporation, San Leandro, Calif.).

  • b The 16S rRNA gene was used as the reference gene for standardization.