TABLE 1.

Genes whose mRNA levels increased exclusively in PQ-treated cells and in a soxR-dependent manner

Gene no./nameP. aeruginosa wt treated with:P. aeruginosa ΔsoxR treated with:P. aeruginosa ΔsoxR/pSoxR treated with PQ (fold change by RT-PCR)
PQH2O2PQH2O2
Fold change by arrayaFold change by RT-PCRbFold change by arrayFold change by RT-PCRFold change by arrayFold change by RT-PCRFold change by arrayFold change by RT-PCR
PA227420.7 ± 3.435.9 ± 0.31.4 ± 0.20.9 ± 0.31.0 ± 0.11.3 ± 0.51.2 ± 0.22.8 ± 0.378.0 ± 0.2
PA37183.4 ± 0.26.5 ± 0.11.1 ± 0.11.0 ± 0.40.6 ± 0.11.8 ± 0.31.1 ± 0.40.7 ± 0.473.5 ± 0.8
PA4205/mexG16.5 ± 2.67.1 ± 0.51.4 ± 0.30.4 ± 0.31.1 ± 0.20.4 ± 0.51.1 ± 0.42.1 ± 0.541.2 ± 0.8
PA4206/mexH12.3 ± 1.614.2 ± 0.41.5 ± 0.20.9 ± 0.31.3 ± 0.31.6 ± 0.51.0 ± 0.21.7 ± 0.231.3 ± 0.2
PA4207/mexI9.4 ± 1.45.8 ± 0.31.5 ± 0.20.9 ± 0.30.9 ± 0.10.7 ± 0.41.0 ± 0.22.2 ± 0.313.0 ± 0.3
PA4208/opmD9.0 ± 1.45.9 ± 0.61.3 ± 0.11.3 ± 0.70.9 ± 0.11.2 ± 1.51.0 ± 0.21.4 ± 1.58.9 ± 2.5
  • a Fold change values were calculated as the ratio of the means of gene signals of PQ- or H2O2-treated and untreated cultures. Fold change values are shown with standard errors. Each mean was derived from six arrays.

  • b Fold changes values were calculated as described in Materials and Methods. Values represent means of triplicates ± standard errors.