TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmidGenotype or descriptionReference or source
Strains
    Escherichia coli DH5-αF φ80dlacZΔM15Δ(lacZYA-argF)U169 deoR recA1 endA1 hsdR17 phoA supE441-thi-1 gyrA96 relA1Gibco-BRL
    Brucella abortus
        2308Virulent challenge strainLaboratory stock
        Hfq32308 hfq::aphA46
        MEK22308 sodC::catThis study
        MEK2CmMEK2 carrying pJG22This study
        MEK2Cm*MEK2 carrying pMEK15This study
Plasmids
    pUC9Cloning vector; ColE-1 origin of replication; ampicillin resistanceGibco-BRL
    pUC18Cloning vector; ColE-1 origin of replication; ampicillin resistanceGibco-BRL
    pBluescriptII KS+Cloning vector; ColE-1 origin of replication; ampicillin resistanceStratagene
    pBBR1MCS-4Cloning vector; broad-host range; moderate copy number (∼10 copies per cell); ampicillin resistance33
    pMR10Cloning vector; broad-host range; low copy number (2 to 4 copies per cell); kanamycin resistanceNCBI accession no. AJ606312
    pBlue-Cm2945-bp PCR amplified cat gene from pBC-SK+ cloned into pBluescriptII KS+47
    pBA233.2-kb Sau3A fragment containing sodC cloned into the BamHI site of pUC935
    pBA23.102-kb HindIII/EcoRV fragment containing sodC cloned into pBluescriptII KS+This study
    pBA23.10::catDerivative of pBA23.10 in which a 226-bp SmaI/NaeI fragment internal to the sodC coding region has been replaced with the cat gene from pBlue Cm2This study
    pMEK152-kb EcoRV/HindIII fragment containing sodC cloned into pBBR1MCS-4This study
    pJG222-kb EcoRI/XhoI fragment containing sodC cloned into pMR10This study