TABLE 2.

Quantitation of the relative numbers of T. pallidum cells in lesions by using real-time PCRa

Passive immunization mediumAvg. no. of T. pallidum DNA copies/μg rabbit DNA ± SE
Day 20Day 25
NRS4.55 × 106 ± 5.9 × 1053.93 × 106 ± 1.1 × 106
IRS3.18 × 105 ± 1.0 × 1051.09 × 106 ± 4.5 × 106
C MAb2.16 × 106 ± 3.7 × 1054.67 × 106 ± 1.3 × 106
M131b7.14 × 105 ± 2.2 × 1055.03 × 106 ± 1.3 × 106
  • a Two representative sites from each animal were biopsied at days 20 and 25 postchallenge for quantitation of T. pallidum DNA copies by real-time PCR. Each sample was assayed three times, and the results for each passively immunized group are presented as the average number of T. pallidum DNA copies per μg of rabbit DNA ± the standard error.

  • b Sites on the M131 passively immunized animals where no lesions appeared showed fewer than 300 T. pallidum DNA copies per μg of rabbit DNA. These values were not included in the DNA copy numbers for the M131 passively immunized animals that developed atypical lesions presented in this table.