TABLE 3.

Results of in vivo competition assay between IMT5155 NalR and its mutants

MutantGene/ homologueCIa in vitroCI of lungCI of liverCI of heartCI of spleenCI of kidney
M03A03 kpsM 1.563.5 × 10−1<1.0 × 10−3<1.0 × 10−3<1.0 × 10−31.5 × 10−1
M18H02 kpsS 5.1 × 10−14.2 × 10−2<1.0 × 10−3<1.0 × 10−31.7 × 10−1<1.0 × 10−3
M03G02 wcaE 8.0 × 10−1<1.0 × 10−3b2.0 × 10−49.0 × 10−31.2 × 10−1
M12D10 waaW 7.0 × 10−12.6 × 10−11.05.1 × 10−22.6 × 10−11.5 × 10−1
M03D10 waaL 6.0 × 10−15.0 × 10−12.5 × 10−11.0 × 10−15.1 × 10−21.2 × 10−1
M00G10None1.671.6 × 10−21.0 × 10−16.3 × 10−3b2.7 × 10−1
M04E08 sitB 3.2 × 10−18.3 × 10−22.1 × 10−14.8 × 10−21.3 × 10−28.8 × 10−2
M11B04 chuA 9.6 × 10−16.45 × 10−21.8 × 10−14.0 × 10−24.12 × 10−21.5 × 10−1
M17F12 mppA 4.6 × 10−16.6 × 10−13.3 × 10−11.02.0 × 10−17.0 × 10−1
M19A01 narK 1.403.8 × 10−14.7 × 10−23.2 × 10−24.0 × 10−14.8 × 10−1
M19B12None6.2 × 10−15.4 × 10−11.26.1 × 10−12.5 × 10−11.0
M08E08 sbmA 6.4 × 10−12.0 × 10−11.4 × 10−12.2 × 10−12.5 × 10−1b
M00C03 r3 5.2 × 10−15.4 × 10−16.1 × 10−11.32.8 × 10−11.1
M04C10 tktA 1.7 × 10−15.51 × 10−16.0 × 10−41.7 × 10−13.9 × 10−39.6 × 10−2
M21C03 ycjM 1.085.3 × 10−11.7 × 10−16.12 × 10−24.6 × 10−13.6 × 10−1
M11E02 malX 1.053.1 × 10−22.2 × 10−11.6 × 10−23.8 × 10−12.1 × 10−2
M13H03None8.0 × 10−12.8 × 10−23.1 × 10−1b2.3 × 10−12.9 × 10−1
M18E07 ychF 5.8 × 10−13.1 × 10−21.42 × 10−2<1.0 × 10−41.5 × 10−21.8 × 10−2
M18D02None2.079.0 × 10−22.9 × 10−12.2 × 10−16.4 × 10−28.5 × 10−1
  • a CI, competitive index. Values > 1 indicate mutant outcompeted wild type, values <1 indicate wild type outcompeted mutant; mutants being outcompeted up to 10-fold were evaluated as slightly, up to 100-fold as moderately, and more than 100- fold as highly attenuated. In vivo competitive indices (CIs) are the average of results gained with two to four animals.

  • b —, not tested.