TABLE 1.

Median cytokine plasma levels and gene expression levels in relation to the housekeeping gene in PBMC of patients with T1R, nonreactional patients, and healthy controls

CytokineMedian level ina:
Patients with T1RNonreactional patientsHealthy controls (n = 7)
Before prednisolone (n = 14)1 month (n = 14)6 months (n = 8)Before MDT (n = 11)1 month (n = 7)
Plasma levels
    TNF-α0 (0, 0)0 (0, 42)0 (0, 91)0 (0, 192)0 (0, 154)64 (0, 149)
    IL-100 (0, 76)0 (0, 81)33 (0, 78)0 (0, 123)0 (0, 127)0 (0, 37)
    TGF-β1(active)327 (0, 596)331 (0, 640)314 (0, 703)325 (0, 603)319 (0, 481)309 (0, 407)
    TGF-β1(total)7,251 (2,329, 18,548)3,134 (1,825, 9,299)b3,719 (18,471, 6,299)11,142 (4,124, 26,978)7,392 (3,461, 18,496)4,071 (3,125, 5,072)c
mRNA levels
    TNF-α0.6 (0.2, 2.1)0.7 (0.3, 1.8)0.6 (0.2, 6.3)0.4 (0.2, 5.1)0.5 (0.3, 0.5)0.4 (0.3, 1.1)
    IL-1β1.2 (0.1, 8.5)1.4 (0.2, 5.8)0.7 (0.1, 6.7)0.8 (0.2, 7.6)0.7 (0.1, 1.0)0.3 (0.1, 1.8)
    IL-1012.9 (3.4, 49.3)46.1 (6.7, 143.4)d14.5 (4.1, 37.5)17.9 (2.7, 45.3)13.1 (4.8, 26.9)8.6 (4.3, 44.4)
    TGF-β12.4 (1.9, 5.8)2.7 (1.5, 5.7)3.9 (1.7, 11.3)3.1 (1.2, 4.9)2.9 (2.3, 4.6)7.3 (3.1, 11.8)e
  • a The cytokine plasma levels are expressed in picograms per milliliter, and the gene expression levels are expressed as the ratio of the number of cytokine mRNA copies to the number of HPRT mRNA copies. The values in parentheses are 5th and 95th percentiles.

  • b Significantly (P < 0.01) lower than the level before prednisolone treatment.

  • c Significantly (P < 0.05) lower than the level for nonreactional patients before MDT.

  • d Significantly (P < 0.001) higher than the level before prednisolone treatment.

  • e Significantly (P < 0.01) higher than the level for T1R and nonreactional patients before treatment.