TABLE 1.

Oligonucleotide primers and LightCycler hybridization probes

Target geneGenBank accession no.PrimerPrimer sequenceaPurpose
asp23 S76213 T7-aspTAATACGACTCACTATAGGGAGAGCATACGACAATCAAACTGGStandard construction
asp886TAAAATGGTGTGCCGAGCTTStandard construction
asp493FAAAATTGCTGGTATCGCTGCRT-PCR, Northern probe
asp848RTGTAAACCTTGTCTTTCTTGGTRT-PCR, Northern probe
clfA Z18852 T7-clfTAATACGACTCACTATAGGGAGAGGCGTGGCTTCAGTGCTTGTAStandard construction
clf725CTAATTCCTCCGCATTTGTATTGStandard construction
clf382FCGGTTTTGGACTACTCAGCART-PCR
clf653RTAGCTTCACCAGTTACCGGCRT-PCR
clfFL1GGCGCAAAATCCAGCACAACAGGAA-FHybridization probe
clfLC1Red640-CGACACAATCATCATCAACAAATGCAAC-phHybridization probe
clfU2GGCGTGGCTTCAGTGCTTGTANorthern probe
clfL2CACCAGTTACCGGCGTTTCTTCNorthern probe
coa X17679 T7-coaTAATACGACTCACTATAGGGAGATAGAAGGTCTTGAAGGTAGCStandard construction
coa1624GGCTTATTGAATCTTGGTCTCGCTStandard construction
coaFAGGTCTTGAAGGTAGCTCATRT-PCR
coaRGTTGTATTCACGGATACCTGTART-PCR
coaFLCTTGATTCTCCTTGAGTACCTTTTAAC-FHybridization probeb
coaLCRed640-TTGATTCAGTACCTTGTGGTTTTATT-phHybridization probe
coa35FTAGATTGGGCAATTACATTTNorthern probe
coa36RACCTTTTCCAACAACCTATGNorthern probe
fnbA J04151 fnb1171ATTGAGACATTTAATAAAGCGART-PCR
fnb1667TTTTGAATAATCGGACCATTRT-PCR
fnbAFTGCAAATACGACAGATACTTNorthern probe
fnbARTTGGCCACCTTCATAACCTANorthern probe
gyrB D10489 T7-gyrTAATACGACTCACTATAGGGAGATTATGGTGCTGGGCAAATACAStandard construction
gyr864GTACGATTTAATACCGCCCTCATAStandard construction
gyr297FTTAGTGTGGGAAATTGTCGATAATRT-PCR
gyr574RAGTCTTGTGACAATGCGTTTACART-PCR
gyrFL1ATTTTAACTGTTTTACATGCTGGTGGTAA-FHybridization probe
gyrLC1Red640-TTTGGCGGTGGCGGATACA-phHybridization probe
hla X01645 T7-hlaTAATACGACTCACTATAGGGAGA AGAAAATGGCATGCACAAAAAStandard construction
hla670TGTAGCGAAGTCTGGTGAAAAStandard construction
hla90AGAAAATGGCATGCACAAAAART-PCR, Northern probe
hla488TATCAGTTGGGCTCTCTAAAART-PCR, Northern probe
hlaFL1CAGGAAAAATTGGCGGCCTT-FHybridization probe
hlaLC1Red640-TTGGTGCAAATGTTTCGATTGG-phHybridization probe
saeR AJ556794 T7-saeTAATACGACTCACTATAGGGAGAAACTGCCAAAACACAAGAStandard construction
saeR2CCATTATCGGCTCCTTTCAStandard construction
saeU4CCATTTACGCCTTAACTTTART-PCR
saeR4TAGTCATATCCCCAAACTTRT-PCR
saeFL2GTGACTGTAAATGGTCACGAAGT-FHybridization probe
saeLC2Red640-CCTATGCGTATTAAGGAATTTGA-phHybridization probe
sae1980FTGGTCACGAAGTCCCTATGCNorthern probe
sae2458RTGCTTGCGTAATTTCCGTTAGNorthern probe
ORF4 AJ556794 T7-ORF4TAATACGACTCACTATAGGGAGACAAATTGAAGAAATGAGGAGTTAStandard construction
ORF358FCAAATTGAAGAAATGAGGAGTTART-PCR, Northern probe
ORF616RACCTTTTGATGATTTGTAGTTAGRT-PCR, standard construction, Northern probe
sigB Y09929 sigB2734AAAGAACACCAAGAAAATAAGNorthern probe
sigB3375TAAACCGATACGCTCACCTGTNorthern probe
  • a F, fluorescein; Red640, LightCycler-Red 640-N-hydroxysuccinimide ester; ph, 3′-phosphate.

  • b LightCycler hybridization probes coaFL and coaLC were designed by TIB Molbiol, Berlin, Germany.