TABLE 1.

Primers

PurposeSense primerAntisense primerSize (bp)
tprI transcriptional analysis5′-GACCCTGCCGATGCAGGTAAT5′-TAAGCACGATGTCCGACTGACT336
tprI sequencing5′-CCTTTCCAATGGTGCCTGGG5′-CGAAGCCATACTTGCTG
5′-ACATCGTGCTTATCATGCT5′-TTGAACCGGTCCAGCCGTG
tprI cloninga
    Full-length TprI (aa 18-609)5′-GGAATTCATATGGCTTCTGGTTATGCAGGCGT5′-GCCCAAGCTTCCAAACCACCTTCACCCC1,799
    Amino terminus (aa 18-281)5′-GGAATTCATATGGCTTCTGGTTATGCAGGCGT5′-GCCCAAGCTTCTGGTGTTGGTTACCGGC814
    Central region (aa 282-393)5′-GGAATTCATATGGACCCTGCCGATGCA5′-GCCCAAGCTTTAAGCACGATGTCCGACTGACT358
    Carboxyl terminus (aa 394-609)5′-GGAATTCATATGCTGCTAACCTGGCTTGAG5′-GCCCAAGCTTCCAAACCACCTTCACCCC664
  • a Underlined sequences represent NdeI (CATATG) and HindIII (AAGCTT) restriction sites in sense and antisense primers, respectively.