TABLE 1.

Impaired capacity to produce fibrin in warfarin-treated mice

Treatment and Timea (min)Fibrin formationb
Nonec
    10>250, >250, >250, >250, >250
    30>250, >250, >250, >250, >250
    90>250, >250, >250, >250, >250
Warfarind
    100, 0, 0, 0, 0
    300, 0, 0, 60, >250
    900, 50, >250, >250, >250
Fib KOe
    100, 0, 0, 0, 0
    300, 0, 0, 0, 0
    900, 0, 0, 0, 0
Heparinf
    100, 0, 0, 0, 0
    300, 0, 0, 0, 0
    900, 0, 0, 0, 0
  • a Time at which further spontaneous fibrin formation was quenched by addition of EDTA-heparin-containing extraction buffer.

  • b Ex vivo fibrin formation in whole blood collected by cardiac puncture after euthanasia by carbon dioxide asphyxiation. Data from five individual animals/group are presented as nanograms of fibrin per 10 μl of whole blood.

  • c Wild-type C57BL/6 mice.

  • d Wild-type C57BL/6 mice treated with warfarin for 8 days.

  • e Fib KO, fibrin(ogen)-deficient mice.

  • f C57BL/6 mice treated with 500 U of heparin i.v. just prior to euthanasia.