TABLE 1.

Strains, plasmids, and primers used in this study

Strain, plasmid, or primerDescriptionSource or reference
Strains
    E. coli TOP10Cloning strainInvitrogen
    M. catarrhalis O35EWild-type strain46
    M. catarrhalis O35E705Strain with EZ::TN transposon insertion in kdtA geneThis study
    M. catarrhalis O35EkdtAkdtA knockout mutant strainThis study
Plasmids
    pCR2.1TOPO TA cloning vectorInvitrogen
    pBluescript II SK(+)Cloning vectorFermentas
    pUC4kKanamycin resistance cassetteAmersham
    pCRAkdtA cloned into pCR2.1This study
    pSAEcoRI-SalI kdtA fragment cloned into SK(+)This study
    pSAKEcoRI-blunted kanamycin resistance cassette inserted into blunted HindIII site of pSAThis study
Primers
    315′-GAA ACC TGA GAG GTA ACT GCA GCC AGT T-3′This study
    325′-GTA TCA TTG CTC AAG ATG CCA GCT CTG C-3′This study
    335′-ATG GAC GGG CAA ATC AGG TGC TGT ATT G-3′This study
    345′-AGC CAT GAA GAT TTG GTG-3′This study
    355′-CTC GTC GAC TTG TCG CAC AAG CAA CGC-3′ (SalI site underlined)This study
    365′-CTC GAA TTC CGT GAC AGT AAT GGT GAA-3′ (EcoRI site underlined)This study