TABLE 2.

Predicted lipid A compositions of LOS preparation from O35E and O35EkdtA mutant based on MALDI-TOF MS analysis

SourceIon m/zPredicted lipid A compositionaRelative intensityb (%)
ObservedCalculated
O35E2,028.302,029.19P2-PEA2-GlcNAc2-3-OHC12:04-C10:02-C12:0124
1,905.351,906.18P2-PEA1-GlcNAc2-3-OHC12:04-C10:02-C12:01100
1,825.391,826.18P1-PEA1-GlcNAc2-3-OHC12:04-C10:02-C12:0132
1,782.341,783.17P2-GlcNAc2-3-OHC12:04-C10:02-C12:0123
1,751.201,752.04P2-PEA1-GlcNAc2-3-OHC12:04-C10:01-C12:0129
O35EkdtA1,905.281,906.18P2-PEA1-GlcNAc2-3-OHC12:04-C10:02-C12:0128
1,782.271,783.17P2-GlcNAc2-3-OHC12:04-C10:02-C12:01100
1,628.271,629.03P2-GlcNAc2-3-OHC12:04-C10:01-C12:0129
1,600.101,601.00P2-GlcNAc2-3-OHC12:04-C10:0235
1,568.991,569.87P2-PEA1-GlcNAc2-3-OHC12:04-C10:0129
1,456.141,456.89P2-GlcNAc2-3-OHC12:04-C12:01 (minus H2O)30
1,445.971,446.86P2-GlcNAc2-3-OHC12:04-C10:0152
1,430.091,430.87P2-GlcNAc2-3-OHC12:03-C10:01-C12:0127
  • a P, phosphate moieties; GlcNAc, N-acetylglucosamine; 3-OHC12:0, 3-hydroxydodecanoyl acyl chain; C12:0, dodecanoyl acyl chain; C10:0, decanoyl acyl chain.

  • b The highest value of each strain represents 100% and others compared with the highest one.