Staphylococcus aureus
Host Response and InflammationmRNA Transcriptome Analysis of Bone in a Mouse Model of Implant-Associated Staphylococcus aureus OsteomyelitisTo investigate the molecular pathogenesis of bone with osteomyelitis, we developed implant-associated osteomyelitis (IAOM) models in mice. An orthopedic stainless pin was surgically placed in the right femoral midshaft of mice, followed by an inoculation of Staphylococcus aureus into the medullary cavity.
Cellular Microbiology: Pathogen-Host Cell Molecular InteractionsStaphylococcus aureus Tet38 Efflux Pump Structural Modeling and Roles of Essential Residues in Drug Efflux and Host Cell InternalizationThe Staphylococcus aureus Tet38 membrane protein has distinct functions, including drug efflux and host cell attachment and internalization mediated by interaction with host cell CD36. Using structural modeling and site-directed mutagenesis, we identified key amino acids involved in different functions.
MinireviewPolymicrobial Interactions in the Urinary Tract: Is the Enemy of My Enemy My Friend?The vast majority of research pertaining to urinary tract infection has focused on a single pathogen in isolation and predominantly Escherichia coli. However, polymicrobial urine colonization and infections are prevalent in several patient populations, including individuals with urinary catheters.
Molecular PathogenesisThe Increased Accumulation of Staphylococcus aureus Virulence Factors Is Maximized in a purR Mutant by the Increased Production of SarA and Decreased Production of Extracellular ProteasesMutation of purR was previously shown to enhance the virulence of Staphylococcus aureus in a murine sepsis model, and this cannot be fully explained by increased expression of genes within the purine biosynthesis pathway. Rather, the increased production of specific S. aureus virulence...
MinireviewRecalcitrant Staphylococcus aureus Infections: Obstacles and SolutionsAntibiotic treatment failure of Staphylococcus aureus infections is very common. In addition to genetically encoded mechanisms of antibiotic resistance, numerous additional factors limit the efficacy of antibiotics in vivo.
Bacterial InfectionsAnalysis of Staphylococcus aureus Transcriptome in Pediatric Soft Tissue Abscesses and Comparison to Murine InfectionsA comprehensive understanding of how Staphylococcus aureus adapts to cause infections in humans can inform development of diagnostic, therapeutic, and preventive approaches. Expression analysis of clinical strain libraries depicts in vitro conditions that differ from those in human infection, but low bacterial burden and the requirement for reverse...
Bacterial InfectionsThree-Dimensional In Vitro Staphylococcus aureus Abscess Communities Display Antibiotic Tolerance and Protection from Neutrophil ClearanceStaphylococcus aureus is a prominent human pathogen in bone and soft-tissue infections. Pathophysiology involves abscess formation, which consists of central staphylococcal abscess communities (SACs), surrounded by a fibrin pseudocapsule and infiltrating immune cells. Protection against the ingress of immune cells such as neutrophils, or tolerance to antibiotics,...
Host Response and Inflammation | SpotlightDifferential Induction of Type I and III Interferons by Staphylococcus aureusStaphylococcus aureus is a leading cause of bacterial pneumonia, and we have shown previously that type I interferon (IFN) contributes to the pathogenesis of this disease. In this study, we screened 75 S. aureus strains for their ability to induce type I and III IFN. Both cytokine pathways were...
Molecular PathogenesisA Small Membrane Stabilizing Protein Critical to the Pathogenicity of Staphylococcus aureusStaphylococcus aureus is a major human pathogen, and the emergence of antibiotic-resistant strains is making all types of S. aureus infections more challenging to treat. With a pressing need to develop alternative control strategies to use alongside or in place of conventional antibiotics, one...
Molecular PathogenesisStaphylococcus aureus Fatty Acid Kinase FakA Modulates Pathogenesis during Skin Infection via ProteasesStaphylococcus aureus fatty acid kinase FakA is necessary for the incorporation of exogenous fatty acids into the lipid membrane. We previously demonstrated that the inactivation of fakA leads to decreased α-hemolysin (Hla) production but increased expression of the proteases SspAB and aureolysin in vitro, and that the ΔfakA mutant causes...